聚乙二醇α-2a干扰素治疗完全应答HBV感染患者分泌γ干扰素变化的研究

The Study of IFN-γ in Serum and the Secretion of PBMCs in Chronic HBV Infection Patients Who Responsed to Pegylated Interferon α-2a Therapy Completely

目的研究应用聚乙二醇α-2a干扰素治疗后发生完全应答的HBV感染患者血清及外周血单核细胞分泌γ干扰素(IFN-γ)变化的意义。方法抽取应用聚乙二醇α-2a干扰素治疗发生完全应答HBV感染患者应答点及6个月后以及健康对照者、未进行治疗的HBV患者的静脉血,分离血清及单核细胞,检测血清中乙型肝炎病毒表面标志物(HBV-M)、乙型肝炎病毒核酸定量(HBV-DNA)、丙氨酸转移酶(ALT)以及血清中和分别应用抗-CD3、抗-CD28和HBcAb刺激的外周血单核细胞(PBMCs)分泌上清中的IFN-γ。结果与未进行治疗的HBV患者相比,完全应答者血清中及PBMCs分泌的IFN-γ明显下降;与正常对照比较无明显差异。结论应用聚乙二醇α-2a干扰素治疗有效清除病毒的同时,发生完全应答患者分泌IFN-γ的能力恢复正常,有利于减轻炎症反应及肝脏组织的修复。

Objective To study the change of IFN-γ in chronic HBV infection patients who responsed to pegylated interferon a-2a therapy compehely. Methods Separated the peripheral blood monouclear cells （PBMCs）and serum from the blood of HBV infected patients who responsed to pegylated interferon a-2a therapy compeltely or no therapy and health controls, cultured the PBMCs with anti-CD3, anti-CD28 or HBcAb together, tested the ALT, HBV-DNA, HBV-M of patients and the IFN-γ inthe serum and the supernatant of different stimulation. Results The IFN-γ in the serum and the superuatant lowered significantly compared to no therapy patients and had no difference with controls. Conclusion When pegylated interferon a-2a cleared HBV effectivly,the secretion of IFN-γ in whole responsed patients recovered normal which lessened the inflammation of liver and recovered liver tissure.