小分子AGE-肽在小鼠糖尿病肾病发生中的作用

The Effect of Low Molecular Weight AGE-peptide on The Development of Diabetic Nephropathy in Mice

目的：探讨小分子糖基化终产物 Ａ Ｇ Ｅ 肽（ Ａ Ｇ Ｅ Ｐ） 与小鼠糖尿病肾病发生之间的关系。方法：腹腔一次性注射四氧嘧啶４００ ｍｇ·ｋｇ － １ 诱导糖尿病小鼠模型，３０ ｄ 或６０ ｄ 后采集血清，用流动注射分析法测定 Ａ Ｇ Ｅ Ｐ。结果：糖尿病小鼠血清 Ａ Ｇ Ｅ Ｐ３０ ｄ 组和６０ ｄ 组均明显高于对照组（ 均为 Ｐ＜ ０ ．０００ １） ；血清 Ａ Ｇ Ｅ Ｐ 与肌酐、２４ ｈ 尿蛋白定量、尿白蛋白排泄量均呈显著正相关（ Ｐ＜ ０ ．０００ １） 。结论： Ａ Ｇ Ｅ Ｐ 可能在糖尿病肾病的发生发展中起重要作用。

Objective:The experiment was designed to investigate the relationship between low molecular weight advanced glycosylation end products (LMW-AGEs)——AGE?peptide (AGE?P) and diabetic nephropathy (DN) in mice.Method:Diabetic mice were induced by injection of 400 mg·kg -1 alloxan and sacrificed in day 30 and 60,respectively. Serum AGE?P was measured by a flow injection assay. Results:Serum AGE?P levels in diabetic mice were significantly increased in day 30 or 60 compared with the control group ( P <0.000?1). Furthermore, there were positive linear correlation between AGE?P and Cr, 24?h Upro, UAE ( P <0.000?1).Conclusion:AGE?P might be involved in the development of diabetic nephropathy.