通过维生素D_(3)结合蛋白介导的溶血磷脂对人外周巨噬细胞的激活作用

Activation of Human Peripheral Macrophages by Lysophosphatidylcholine with Medium of VD_(3)-Binding Protein

目的：揭示溶血磷脂（ Ｌｙｓｏ Ｐｃ） 在人外周巨噬细胞激活中的作用。方法：用 Ｌｙｓｏ Ｐｃ 处理外周淋巴细胞（ 粘附细胞和非粘附细胞） 后加入维生素 Ｄ３ 结合蛋白（ Ｄ Ｂ Ｐ） ，在３７ ℃、含体积分数为０ ．０５ 的 Ｃ Ｏ２ 中孵育３ ｈ ，测上清液超氧阴离子（ Ｓ Ｏ Ｐ） 浓度。结果：用 Ｌｙｓｏ Ｐｃ 处理后 Ｓ Ｏ Ｐ 浓度明显增高（ Ｐ＜０ ．０１） ；未用 Ｌｙｓｏ Ｐｃ 处理或不加入 Ｄ Ｂ Ｐ， Ｓ Ｏ Ｐ 产生无明显升高（ Ｐ＞ ０ ．５） 。结论： Ｌｙｓｏ Ｐｃ 能通过 Ｄ Ｂ Ｐ 激活巨噬细胞，因此 Ｄ Ｂ Ｐ 是巨噬细胞激活因子前体。

Objective:The aim of the experiment was to demonstrate the effect of lysophosphatidylcholine(Lyso?Pc) in activating human peripheral macrophages.Method:VD 3?binding protein (DBP) was added to human peripheral cells(mixture of adherent and nonadherent cells ) with Lyso?Pc and cultured in CO 2 (φ=0.05) at 37?℃ for 3?h.And then the concentration of superoxide products (SOP) in medium supernatant was determined. Result:The concentration of SOP in medium supernatant was significantly increased ( P <0.01),while that without addition of either DBP or Lyso?Pc did not( P >0.5).Conclusion:Lyso?Pc can activate macrophages with DBP participated.Thus,DBP is the precursor of macrophage activating factor.