阿魏酸酯酶产生菌的筛选及产酶条件的优化

Screening of Feruloyl Esterases Producing Strain and Optimization of the Enzyme Production

从土壤中筛选到1株阿魏酸酯酶活力较高的菌株,通过形态观察,初步鉴定其为曲霉.通过单因素轮换法,对其液态发酵的产酶条件进行优化.结果表明:在查氏培养基中,以质量体积比为6%的麦麸为碳源,质量体积比为5%的硝酸钠为氮源,在30℃下发酵培养6 d后,其酶活力最高可达16.33μkat·L-1.

A strain producing feruloyl esterases was obtained from the soil It was preliminarily identified as Aspergillus sp. according to the morphological characteristics. Production of feruloyl estserses was optimized by single factor method. Results showed that the highest enzyme activity could reach 16. 33 μkat·L^-1 after 6 days at 30℃ in Czapek medium containing 6% （mass/volume） wheat bran and 5% （mass/volume） sodium nitrate.