鼻咽癌组织中潜伏膜蛋白1的表达对癌细胞增殖和凋亡的影响

Effects of LMP 1 expression on neoplastic cell proliferation and apoptosis in nasopharyngeal carcinoma

目的 探讨治疗前鼻咽癌组织中潜伏膜蛋白１（ＬＭＰ１） 的表达对癌细胞增殖和凋亡的影响。方法 收集未经治疗的鼻咽癌活检组织标本５５ 例，采用原位分子杂交和免疫组化检测ＥＢ 病毒编码的小ＲＮＡｓ（ＥＢＥＲｓ）和ＬＭＰ１ 以及癌细胞增殖细胞核抗原指数（ＰＣＮＡｉｎｄｅｘ，ＰＩ） 和癌组织中凋亡相关基因产物ｂｃｌ２ 的表达，以末端标记原位细胞死亡指数（ＴＵＮＥＬｉｎｄｅｘ，ＴＩ）表示癌细胞的凋亡程度。结果 （１）５５ 例未治疗鼻咽癌组织中２７ 例呈ＬＭＰ１ 阳性（４９．１％ ），其ＰＩ中位数为９０ ％ ，高于２８ 例ＬＭＰ１ 阴性病例的８０ ％ ，两者差异具有显著性，Ｐ＜ ０．０５；（２）２７ 例ＬＭＰ１ 阳性病例的ＴＩ中位数为每高倍视野１６．２ 个，高于２８ 例ＬＭＰ１ 阴性病例（１０．４ 个） ，两者差异也具有显著性，Ｐ＜ ０．０５；（３）ＬＭＰ１ 阳性与阴性病例表达ｂｃｌ２ 的癌细胞百分数差异无显著性，Ｐ＞ ０．０５；（４） 鼻咽癌组织中癌细胞的ＰＩ与ＴＩ呈正相关， Ｐ＜０ ．０１ ，但ＴＩ与表达ｂｃｌ２ 的癌细胞百分数却无相关性， Ｐ＞０ ．０５。结论 （１） 鼻咽癌组织中ＬＭＰ１ 阳性者有较高的ＰＩ。

Objective To investigate the effects of LMP 1 expression on neoplastic cell proliferation and apoptosis in untreated nasopharyngeal carcinoma (NPC). Methods Biopsies from 55 untreated NPC cases were collected. EBERs was detected by in situ hybridization. PCNA, LMP 1 and bcl 2 were detected by immunohistochemistry method. Cell apoptosis was demonstrated by TUNEL (TdT mediated dUTP x nick end labeling) kit. Results (1) The positive LMP ① expression rate was 27/55 (49.1%). The median PI (PCNA Index) of LMP 1 positive cases (90.0%) was higher than that of LMP 1 negative cases (80.0%) and the p value was less than 0.05 by rank sum (rs) test. (2) The median TI (TUNEL Index) of LMP 1 positive cases (16.2 per high power field) was also higher than that of LMP 1 negative cases (10.4 per high power field) and the p value was less than 0.05 by rs test. (3) As for the bcl 2 expression percentages, no correlation was found between LMP 1 positive and negative cases. (4) A positive rank correlation existed between PI and TI (rs=0.368 1, P <0.01), but no correlation was found between TI and bcl 2 expression percentage. Conclusions (1) NPC with LMP 1 expression have higher levels of PI, maybe resulting from the promotional effect of LMP 1 on cell proliferation. (2) NPC with LMP 1 expression also have a higher level of TI, indicating that LMP 1 can induce cell apoptosis, which could not be blocked by bcl 2 expression. The authors suggest that LMP 1 expression may exert a dual action on neoplastic cell proliferation and apoptosis in untreated nasopharyngeal carcinoma.