精制冠心方水提液的大孔树脂纯化工艺优选

Study on Refine Technics of Fufang Jingzhiguanxin with Macroporous Resin

目的:研究大孔吸附树脂富集冠心膏水提液中有效成分的工艺条件及参数。方法:以丹酚酸B和芍药苷为指标,采用高效液相法进行含量测定,优选大孔树脂纯化的最佳工艺。结果:D101大孔吸附树脂纯化效果较好,其最佳工艺的条件为径高比1:7,上样液生药质量浓度0.2 g·mL-1,药液最大上样体积为树脂量的8倍,洗脱剂40%乙醇,解析速率3 BV·h-1,洗脱剂用量4 BV。结论:D101型大孔吸附树脂纯化冠心膏水提液的最佳工艺稳定、可行。

Objective：To investigate the purification processes of salvianolic acid B and paeoniflorin from Fufang Jingzhiguanxin with macroporous adsorption resin.Method：To measure the content of salvianolic acid B and paeoniflorin with HPLC.To evaluate the optimal macroporous adsorption resin.Result：The optimal macroporous adsorption resin was D-101.The optimal purification conditions：The sample concentration was 0.2 g.mL-1.The diameter height ratio was 1 ∶ 7.The sample volume was 8 BV.The eluant was 40% ethanol.The elution flow rate was 3 BV.h-1.The ethanol consumption was 4 BV.Conclusion：D-101 has a good purification effect under the above conditions.