无患子皂苷的大孔吸附树脂分离纯化工艺

Study on Isolation and Purification of Saponin from Sapindaceae with Macroporous Resin

目的:筛选适合分离纯化无患子总皂苷的大孔吸附树脂并确立纯化工艺参数。方法:以大孔吸附树脂对常春藤皂苷元保留率为考察指标,选用4种型号的大孔吸附树脂进行纯化。结果:最佳条件是采用D-101型大孔吸附树脂,洗脱乙醇体积分数为50%,乙醇用量为5 BV。结论:D-101树脂在所确定的工艺条件下,可较好地分离纯化无患子皂苷。

Objective：To screen the suitable resin for purification of saponin from sapindaceae and to establish the optimum technological parameters of purification.Method：According to the retention rate of the hederagenin,four types of macroporous resin were used to purify the saponin from sapindaceae.Result：The best conditions was：D-101 of macroporous resin;the concentration of ethanol eluate was 50%;the usage of ethanol was 5 BV.Conclusion：D-101 of macroporous resin showed good capability of isolation and purification of saponin from sapindaceae.