淫羊藿中淫羊藿苷和总黄酮的闪式提取工艺

Study on Flash-type Extraction Process for Total Flavonoids and Icariin from Herba Epimedii

目的:研究闪式提取法对淫羊藿中淫羊藿苷和总黄酮的最佳提取工艺条件。方法:以淫羊藿苷和总黄酮含量为指标,通过正交试验得出闪式提取法提取其淫羊藿苷和总黄酮的最优条件。结果:闪式提取法各因素对总黄酮和淫羊藿苷提取效果的影响程度依次为:提取次数>提取时间>液固比>乙醇体积分数,最佳提取工艺为:加25倍量50%乙醇提取3次,每次10 min。结论:闪式提取法是一种高效、快速提取淫羊藿中总黄酮和淫羊藿苷的方法。

Objective：To study and optimize the extraction and preparation technics of icariin and total flavonoids by orthogonal design.Method：The best conditions of extracting icariin and total flavonoids from Herba Epimed by flash-type extraction were screened by using method of orthogonal design and selecting the amount of icariin and total flavonoids as index.Result：The effect influencing factors of flash-type extraction were as follows：extraction times 〉 duration of extraction 〉 ratio of solid to liquid 〉 concentration of ethanol.The optimum extraction condition obtained was：10 minutes and 3 times,ratio of solid to liquid 1∶ 25,50% ethanol.Conclusion：Flash-type extraction was found to be an efficient and rapid method for extracting total flavonoids and icariin from Herba Epimedii.