金匮肾气丸对腺嘌呤致不育大鼠精子质量与肾脏功能的影响

Effect of Jinkui Shenqi Pill on Sperm Quality and Renal Function in Rats Adenine-induced Infertility

目的:观察金匮肾气丸对腺嘌呤致雄性不育大鼠精子质量及肾功能的影响。方法:30只成年雄性SD大鼠随机分为空白组、模型组、金匮肾气丸组。应用自动精子质量分析仪检测实验大鼠的精子质量(精子密度、活率及活动度);应用放射免疫法测定血清睾酮(T)、雌二醇(E2)、促黄体激素(LH)和促卵泡激素(FSH)含量;自动生化分析仪分析血清尿素氮(BUN)、肌酐(Scr)含量;HE染色、光镜观察肾脏组织变化,透射电镜观察实验大鼠睾丸中精子鞭毛密度。结果:金匮肾气丸能明显增加大鼠精子密度、活率及活动度,提高大鼠血清T含量,降低E2,LH,FSH含量;降低血清BUN和Scr含量。结论:金匮肾气丸可提高模型大鼠的精子质量,改善肾功能。其提高精子质量可能是通过刺激间质细胞产生T及降低异常升高的E2这一间接作用实现的。

Objective：To observe the influence of Jinkui Shenqi Pill on sperm quality and renal function in rats with adenine-induced infertility.Method：Thirty adult male SD rats were randomly divided into Jinkui Shenqi Pill group,model group and control group.Sperm qualities of experimental rats were detected by automatic sperm quality analyzer,and contents of testosterone（T）,estradiol（E2）,luteinizing hormone（LH） and follicle-stimulating hormone（FSH） were measured by radioimmunoassay.Contents of BUN and Scr were ested by automatic biochemical analyzer.Renal tissue was observed by optical microscope.Testis was observed by transmission electron microscopy.Result：Jinkui Shenqi Pill could significantly improve symptoms of Shen-yang deficiency in adenine-induced infertility rats,and it could improve the sperm quality,including sperm density,sperm motility and sperm activity.It could increase content of T obviously,lower contents of E2,LH and FSH,lower contents of BUN and Scr.And it can better the damaged kidney tissue in model rats.Conclusion：Jinkui Shenqi pill can improve sperm quality significantly and improve function of kidney.And the mechanism of improving sperm quality may be related to the increase in content of T by stimulating the interstitial cells and lowering level of E2.