腹泻患者中细菌L型的检测分析

Detection and Analysis of L-type Bacteria in Patients with Diarrhea

目的探索细菌L型检测在腹泻患者病原检索中的必要性,耐药性。方法对监测点腹泻样品用常规方法作病原检索同时用高渗培养方法进行细菌L型检测,对L型菌回复返祖,分别对L型菌和返祖菌作药敏试验。结果病原菌阳性率9.22%,细菌L型阳性率1.83%,37株L型菌全部回复成返祖菌。L型菌主要对诺氟沙星(78.38%),头孢噻肟(72.97%)敏感,对氨苄西林(83.78%),四环素(70.27%)耐药;返祖菌主要对诺氟沙星(64.86%),复方新诺明(62.16%)敏感,对利福平(94.59%),头孢噻吩(83.78%)耐药。药物种类敏感性差异率100%。结论腹泻患者中存在L型菌,病原菌与L型菌菌谱不一致,L型菌和返祖菌的药物敏感性不同,为临床提供用药依据。

[Objective]To study the necessity of L-type bacteria detection in pathogen retrieval of patients with diarrhea,and its drug resistance.[Methods]The pathogen retrieval was performed by conventional methods in diarrhea samples from monitoring point,meanwhile,the L-type bacteria detection was conducted by hypertonic culture method.L-type bacteria were reverted,and L-type bacteria and reversion bacteria were detected by the drug sensitivity test.[Results]The positive rate of pathogen bacteria and L-type bacteria was 9.22% and 1.83% respectively.37 strains of L-type bacteria all revert to reversion bacteria.L-type bacteria are mainly sensitive to Norfloxacin（78.38%） and Cefotaxime（72.97%）,and are resistant to Ampicillin（83.78%） and Tetracycline（70.27%）.The reversion bacteria are mainly sensitive to Norfloxacin（64.86%） and compound Sulfamethoxazole（62.16%）,and are resistant to Rifampicin（94.59%） and Cephalothin（83.78%）.The sensitivity differential rate of drug varieties is 100%.[Conclusion]L-type bacteria exist in diarrhea patients,the distribution of pathogen bacteria and L-type bacteria is different,the drug sensitivity of L-type bacteria and reversion bacteria is different,all of which provide the basis of medication for the clinic.