草原白蘑菌种分离及菌丝体生长因子试验

Isolation of Tricholoma mongolicum and test of growth factor on mycelium

草原白蘑是我国宝贵的珍稀食用菌资源,由于草场退化及人为过度采集,产量不断下降。为了保护草原白蘑且对其进行更好的开发利用,现对其生长因子进行研究。结果表明：选取菌褶处的组织进行分离菌丝体,萌发率在35%。草原白蘑的菌丝体生长的最佳碳源为蔗糖;最佳氮源为酵母膏;当碳氮比为20：1~30：1时,菌丝生长较快。最适合菌丝体生长的温度为22~28℃,最适pH为6.5~7.0。Vc、VB6和α-萘乙酸能较好地促进菌丝体的生长。

Tricholoma mongolicum is one of the rare and precious mushroom resources.As grassland degradation and human over-harvesting,production are decline.in order to protect Tricholoma mongolicum and exploit this resources better,different nurtrition for growth of Tricholoma mongolicum mycelium were studied.The result indicates that：lamella are select to separate mycelium,the rate of bourgeon is about 35%.Saccharose is best carbon source,and the best nitrogen source is yeast extract;when the carbon and nitrogen ratio is 20：1～30：1,mycelium is growing well.The optimum temperature is 22～28 ℃,and the optimum pH is 6.5～7.0.Vc,VB6,and α-NAA can better promote the growth of mycelium.