纳米硒-氨基酸溶胶的抗氧化作用

Antioxidant activity of selenium nanoparticle-amino acid sols

采用Fenton体系法、邻苯三酚自氧化法、DPPH法和ORAC法评价纳米硒-谷氨酸、纳米硒-甘氨酸和纳米硒-丙氨酸溶胶的抗氧化能力。结果表明:3种纳米硒-氨基酸溶胶清除OH-.能力在一定范围内随溶胶浓度的增加而增加,然后趋于平缓;溶胶清除O2-.能力与溶胶浓度以及其中纳米硒(nanoSe0)的量均呈现正相关,其溶胶的IC50值分别为(57.5±7.5)μmol/L(nanoSe0-Glu)、(6.78±0.23)μmol/L(nanoSe0-Gly)、(32.3±6.9)μmol/L(nanoSe0-Ala);3种溶胶都具有较强的清除DPPH.能力,其IC50值分别为(15.5±0.27)μmol/L(nanoSe0-Glu)、(15.3±0.89)μmol/L(nanoSe0-Gly)、(15.2±0.60)μmol/L(nanoSe0-Ala)。ORAC检验结果显示,3种纳米硒-氨基酸溶胶的荧光衰退曲线的延缓面积(AUC)与其浓度呈良好的正相关性,并且nanoSe0-Ala溶胶的总抗氧化能力强于nanoSe0-Glu溶胶和nanoSe0-Gly溶胶。研究结果表明纳米硒-氨基酸溶胶可以有效的清除O2-.、OH-.和DPPH.且总抗氧化活性较强,作为一种有效的抗氧化剂有望应用于食品防腐和食品保健等方面。

The in vitro radical scavenging and antioxidant capacity of selenium nanoparticle（nanoSe0）-amino acid sols,such as nanoSe0-Glu,nanoSe0-Gly and nanoSe0-Ala were evaluated by using different in vitro analytical methods.The experimental results demonstrated that the hydroxyl radical（OH-·） scavenging capacity of nanoSe0-amino acid sols increased with the concentration increasing.NanoSe0-amino acid sols also had an effective superoxide anion free radical（O-2·） scavenging capacity which were positively related with the con-centration of nanoSe0-amino acid sols and nanoSe0.The IC50 values of nanoSe0-amino acid sols were（57.5±7.5） μmol/L（nanoSe0-Glu）,（6.78±0.23） μmol/L（nanoSe0-Gly）,（32.3±6.9） μmol/L（nanoSe0-Ala）,respectively.NanoSe0-amino acid sols scavenged 1,1-2diphenyl-2-pierylhydrazyl free radical（DPPH·） effectively.The IC50 values of nanoSe0-amino acid sols were（15.5±0.27） μmol/L（nanoSe0-Glu）,（15.3±0.89） μmol/L（nanoSe0-Gly）,（15.2±0.60）μmol/L（nanoSe0-Ala）,respectively.In addition,the results of the oxygen radical absorbance capacity（ORAC） showed that the area under curve（AUC） of the nanoSe0-amino acid sol was linearly related to its concentration and the antioxidant capacity of nanoSe0-Ala sol was higher than those of nanoSe0-Gly sol and nanoSe0-Glu sol.As a conclusion,nanoSe0-amino acid sols could scavenge O2-·,OH-·,DPPH· efficiently,it could be used as natural antioxidant component and food preservative agents or nutraceuticals.