一株阿特拉津降解菌的分离鉴定及降解特性

Isolation, identification and characterization of an atrazine-degrading strain

从农药厂废水处理池的活性污泥中分离到一株阿特拉津降解菌X-4,根据其生理生化特性和16S rRNA基因序列相似性分析,将其初步鉴定为节杆菌属(Arthrobacter sp.)。该菌能以阿特拉津为唯一碳氮源生长,42 h内对100 mg/L的阿特拉津降解效果为95.7%,降解阿特拉津的最适温度为30°C,pH为7.0。该菌对多种重金属离子都存在抗性,显示了其在去除阿特拉津和重金属复合污染方面的应用潜力。对其降解基因的初步研究显示,该菌含有trzN、atzB和atzC 3个阿特拉津降解相关基因。

A bacteria strain X-4 capable of degrading atrazine was isolated from the activated sludge from the wastewater treating system of an atrazine manufacturer.It was preliminarily identified as Ar-throbacter sp.according to its physiological biochemical characteristics and the analysis of its 16S rRNA gene sequence.This strain could use atrazine as sole carbon and nitrogen source,and degrade 95.7% of 100 mg/L atrazine within 42 h.The optimal pH and temperature for the degradation were 30 °C and pH 7.0,respectively.Strain X-4 could tolerate many kinds of heavy metals,which showed its po-tential in treating the co-contamination of atrazine and heavy metals.The atrazine-degrading related genes of strain X-4 were the combination of trzN,atzB and atzC.