1,3-二氯苯对芦苇湿地土壤生物活性的影响

Influences of 1,3-dichlorobenzene on biological activity in reed wetland soil

采用传统微生物培养及酶学方法,研究了1,3-二氯苯(1,3-DCB)对芦苇湿地土壤微生物及酶活的影响。结果表明,1,3-DCB处理芦苇湿地土壤对微生物不同类群影响不同,其中对细菌和放线菌生长具有抑制作用,各浓度1,3-DCB处理对放线菌的抑制较为显著,浓度高于120μg/g显著抑制对细菌的生长。1,3-DCB对真菌表现为先刺激后抑制。在实验过程中,1,3-DCB可显著抑制湿地土壤过氧化氢酶和多酚氧化酶的活性,表明这2种酶是1,3-DCB污染芦苇湿地土壤的敏感指标。

With traditional microbiological culture and enzyme method,the effect of 1,3-dichlorobenzene（1,3-DCB） on reed wetland soil microbial and enzyme activities was studied.Results demonstrated that different groups of oil microorganisms have different responses to 1,3-DCB,in which the actinomycetes was significantly inhibited.When the 1,3-DCB concentration was higher than 120 μg/g,the bacterial amounts were significantly inhibited.The soil fungi amounts were initially increased at the beginning of the experiment but inhibited with time.During the experiment,1,3-DCB treatment could significantly inhibit the soil catalase and polyphenol oxidase activity.Results indicated that these two enzymes are sensitive to 1,3-DCB contamination.