四角蛤蜊多糖脱蛋白方法比较

Comparative Study on Deproteinization Methods for Polysaccharide from Mactra veneriformis

目的:优选出四角蛤蜊粗多糖脱蛋白的方法,并对脱蛋白后的产物进行含量测定和活性验证。方法:通过离子交换法、三氯乙酸(TCA)法、絮凝法及盐析法分别对四角蛤蜊粗多糖进行脱蛋白,比较各种方法的脱蛋白效果。结果:离子交换法、TCA法、盐析法脱蛋白效果都较理想,所得除蛋白多糖总糖含量分别为85%、90.96%、83%,絮凝法不适合对四角蛤蜊粗多糖中蛋白质的去除,活性实验表明TCA法和离子交换法所得样品降血糖活性较好,其中TCA法所得样品与模型对照组比较有显著性差异,P<0.05。结论:优选方法合理可行。

Objective：To screen the optimum method for deproteinizing crude polysaccharide from Mactra veneriformis and to determine the polysaccharide content and biological activity of purified product.Methods：The deproteinization of crude polysaccharide from Mactra veneriformisw as comparatively studied using ion exchange,trichloroacetic acid（TAC）method,PEG flocculation and salt fractionation.Results：Ion exchange,trichloroacetic acid（TAC）method and salt fractionation had good deproteinizing effect on crude polysaccharide from Mactra veneriformis,and the polysaccharide contents in the resulting purified products were 85%, 90.96%and 83%,respectively.PEG flocculation was unsuitable for the removal of protein impurities from crude polysaccharide from Mactra veneriformis.Ion exchange and TAC method provided highly effective hypoglycemic products,and the product derived from TAC method displayed a significant difference compared to the model control（P0.05）.Conclusion：The screened methods are feasible in practice.