响应面法优化野艾蒿多糖的超声波提取及其抗氧化性研究

Response Surface Methodology for Optimization of Ultrasound-Assisted Extraction and Antioxidant Evaluation of Polysaccharides from Artemisia lavandulaefolia Leaves and Stems

为确定野艾蒿多糖超声波提取的最佳工艺条件,利用中心组合(Box-Behnken)试验设计原理,采用三因素三水平响应面分析法,获得多元二次线性回归方程,以多糖提取率为响应值作响应面和等高线图,并考察野艾蒿多糖的体外抗氧化活性。结果表明:野艾蒿多糖最佳超声波提取工艺条件为超声时间35min、提取温度70℃、水料比40:1(mL/g),在此工艺条件下,多糖得率6.44%与理论预测值6.71%的相对误差为4.02%,拟合度较好。野艾蒿多糖的总抗氧化能力较强,对DPPH自由基具有一定的清除能力。

To optimize the ultrasound-assisted extraction of polysaccharides from Artemisia lavandulaefolia leaves and stems, a multiple quadratic regression describing the relationship between the extraction rate of polysaccharides and extraction parameters such as extraction time,temperature and ratio of water to material was established using a 3-factor,3-level Box-Behnken statistical design,and response surface and contour plots with the extraction rate of polysaccharides as the response were drawn based on the model.The optimum conditions for extracting polysaccharides from Artemisia lavandulaefolia leaves and stems were determined as follows：ultrasound treatment time 35 min,temperature 70℃and ratio of water to raw material 40 mL/g.Under such conditions,the relative error between the experimental and theoretical extraction rates of polysaccharides was 4.02%, indicating that the goodness of fit of the established regression model is quiet good.Polysaccharides from Artemisia lavandulaefolia leaves and stems had strong total antioxidant capacity and showed scavenging ability towards DPPH free radicals.