玉米须多糖中蛋白质脱除的Sevag与酶法联用工艺优化

Removal of Proteins from Corn Silk Polysaccharide by A Combined Enzymatic-Sevag Method

采用Sevag与酶法联用优化玉米须多糖脱蛋白的工艺。以脱蛋白率和多糖剩余率及综合评分为考察指标。结果表明,最佳工艺为酶底比2.5(mg/L)、温度50℃、振荡时间8min、脱蛋白次数2次。最终多糖纯度由21.88%提高为37.77%,脱蛋白率69.97%多糖,剩余率94.51%。此方法可用于玉米须多糖中蛋白质的脱除。

Enzymatic hydrolysis followed by Sevag treatment was used to develop a combined method for removing proteins in crude corn silk polysaccharide.The enzyme used was papain.Its hydrolysis conditions were optimized by single factor and orthogonal array design methods based on deproteinization rate and the recovery of polysaccharides.The number of repeated Sevag treatment was also investigated.The optimal conditions for deprotenizing crude corn silk polysaccharide were as follows： enzyme-to-substrate ratio of 2.5（mg/L）,temperature of 50℃,oscillation time of 8 min and number of repeated Sevag treatment of 2.Under such conditions,the purity of crude corn silk polysaccharide was increased from 21.88%to 37.77%,and the deproteinizaiton rate and polysaccharide recovery were 69.97%and 94.51%,respectively.