脱脂麦胚棉子糖提取液的脱色

Decolorization of Raffinose Ethanol Solution from Defatted Wheat Germ

为提高棉子糖产品的质量,采用活性炭对棉子糖提取液脱色。先利用单因素试验分别考察活性炭添加量(1～5g/100mL)、脱色温度(40～80℃)、pH值(4.5～5.5)、脱色时间(0.5～2.5h)对脱色效果的影响。而后在此基础上采用三因素三水平响应面分析法,依据回归分析确定较优脱色条件。结果表明,较优的脱色条件为活性炭添加量4g/100mL、脱色温度50℃、pH5.0、脱色时间2h。在此条件下脱色率可达94.6%,且棉子糖损失极小。采用活性炭对脱脂麦胚棉子糖提取液脱色可取得良好效果。

The high content of pigment makes it difficult to crystallize raffinose from its ethanol solution obtained from defatted wheat germ.In the present study,activated charcoal（AC）was used to develop a method for decolorizing raffinose solution,and the decolorization conditions AC dose,temperature,pH and operation time were optimized by single factor experiments,where the effects of the four conditions on decolorization rate were preliminarily,in combination with a response surface design involving 3 factors（AC dose,temperature and pH）at 3 levels.The optimum decolorization conditions were determined as follows：AC dose,4 g/100 mL;temperature,50℃;pH,5.0;and adsorption time,2 h.Under these conditions,the decolorization rate was up to 94.6%,and a very small amount of raffinose was lost.Therefore,active carbon is applicable to decolorization of raffinose.