氨基化聚苯乙烯微球的制备及在克伦特罗抗体纯化中的应用

Preparation of Amino-polystyrene Microspheres and Its Application in Purification of Antibodies against Clenbuterol

进行氨基聚苯乙烯微球制备方法及其应用于克伦特罗抗体纯化的研究。通过分散聚合法制备聚苯乙烯微球,硝基反应成硝基化聚苯乙烯微球,再还原成氨基化微球。并利用电子显微镜及红外光谱仪对微球形态及结构进行表征。成功制备氨基聚苯乙烯微球后,将微球与盐酸克伦特罗分子发生重氮化反应,得到连接克伦特罗分子的微球。将这种微球免疫亲和吸附分离盐酸克伦特罗的抗体。最后将纯化后的抗体通过紫外检测,电泳检测等方法证明所制备的微球用于抗体的纯化可行。

The objectives of this study were to develop a preparation method for amino-polystyrene microspheres and to evaluate its application in purification of antibodies against clenbuterol.Polystyrene was prepared by dispersion polymerization, and converted into polystyrene microspheres by nitration reaction,which were reduced into amino-microspheres.Scanning electron microscopy and infrared spectrometry was used to characterize the shape and structure of microspheres.Aminopolystyrene microspheres were bound with clenbuterol molecules by diazotization reaction.Amino-polystyrene microspheres with clenbuterol were applied in the immunoaffinity adsorption and separation of anti-clenbuterol antibodies.Finally,purified antibodies were detected by UV and electrophoresis assay.We proved that it is feasible to use amino-polystyrene microspheres with clenbuterol prepared in this study to purify anti-clenbuterol antibodies.