QuEChERS-气相色谱法快速检测五十种中药材中九种有机氯农药残留的方法研究

Analysis of Pesticide Residues Using the Quick Easy Cheap Effective Rugged and Safe(QuEChERS) Pesticide Multiresidue Method in Fifty Traditional Chinese Medicine by Gas Chromatography

建立了简便、快速测定五十种中药材中九种农药的多残留检测方法。以体积比95:4:1的乙腈-水-1%乙酸混合溶液作为提取剂,QuEChERS(Quick,Easy,Cheap,Effective,Rugged,Safe)方法进行净化,采用气相色谱法进行检测。结果表明:九种农药在1.0～100ng/mL范围内线性良好,3个添加水平的平均回收率分别为76.0%～114.0%、86.1%～95.7%和82.8%～104.6%,检出限在0.4～1.5μg/kg范围内。该方法快速、简便,并且成本较低。

A simple and rapid method was developed for determining nine pesticide residues in fifty traditional Chinese medicine.Pesticides were extracted with mixed solvents of acetonitrile,water and 1% acidum aceticum（95：4：1,V/V/V）.Extracts were cleaned-up by QuEChERS（Quick,Easy,Cheap,Effective,Rugged and Safe） pesticide multiresidue method,and then detected by gas chromatography with electron capture detection.Good linearity was obtained in the concentration range of 1.0～100 ng/mL.Average recoveries of 9 pesticides at three concentration levels were in the ranges of 76.0%～114.0%,86.1%～95.7% and 82.8%～104.6%,respectively.The limits of detection of 9 pesticides were in the ranges of 0.4～1.5 μg/kg.The method is easy and low-cost.