近红外光谱法同时测定黄芪精口服液中黄芪多糖和黄芪甲苷的含量

Simultaneous Determination of Astragalus Polysacharin and Astragaloside Ⅳ in Huangqijing Oral Liquid by Near Infrared Spectroscopy

应用傅立叶变换近红外光谱仪透射光谱技术对黄芪精口服液中黄芪多糖(APS)和黄芪甲苷(AGS)的含量进行检测分析,采用偏最小二乘回归法(PLS)建立了黄芪精口服液中APS和AGS含量近红外数学校正模型。通过内部交叉验证,确定了模型的最佳变量数,得到了建立模型的最佳参数,并通过预测集对模型进行了外部验证。黄芪多糖的相关系数达到0.9797,交叉验证均方差(RMSECV)为0.153,预测集均方差(RMSEP)为0.178;AGS的相关系数达到0.9412、RMSECV为0.0365,RM-SEP为0.0458。通过对模型进行t检验,在给定显著性水平为0.05的条件下,其测定结果与标准方法的测定结果对比,两者无显著性差异。实验结果表明,近红外光谱法可以快速、准确测定黄芪多糖和黄芪甲苷的含量,有望用于黄芪精口服液的在线检测。

A new method was established to simultaneously determine the astragalus polysacharin and astragalosideⅣ in Huangqijing oral liquid by Fourier transfrom near infrared spectroscopy（FT-NIR） with partial least square（PLS）.The Rank of the model and the optimized parameters of the mathematical model by calibration samples were gotten by internal cross validation.Then,the model was validated by the prediction set.The multicorrelation coefficients（R2） of the model to the astragalus polysacharin and astragaloside Ⅳ were 0.9797 and 0.9412,respectively.The root mean square errors of cross validation（RMSECV） of the model to the astragalus polysacharin and astragaloside Ⅳ were 0.153 and 0.0365,and the root mean square errors of prediction（RMSEP） were 0.178 and 0.0458,respectively.By statistical significance test,the results of determination were compared with those of standard methods with no significant difference at 0.05 level.The result shows that the method proposed can be used to simultaneously determine the astragalus polysacharin and astragaloside Ⅳ in huangqijing oral liquid with high accuracy.