绿原酸的直接荧光分析法及其抗氧化性研究

Study on Direct Fluorescence Analysis and Anti-oxidation of Chlorogenic Acid

绿原酸水溶液能产生微弱荧光,当加入95%的乙醇后,其荧光强度大大增强,基于此,建立了直接测定绿原酸的荧光分析新方法。pH=1.72时,在1.416～14.16μg/mL浓度范围内,绿原酸的荧光强度与其浓度呈良好的线性关系,相关系数r为0.9963;检出限为0.081μg/mL;相对标准偏差为0.54%。该方法灵敏度高且稳定性好,已成功用于金银花和杜仲皮中绿原酸的测定。试验还表明,含有绿原酸的金银花和杜仲皮等中草药有良好的抗氧化性。

Chlorogenic acid in aqueous solution produces a weak fluorescence,while the fluorescence intensity is greatly enhanced in 95% ethanol.Based on this fact,a new method for the determination of chlorogenic acid was established.At pH=1.72,chlorogenic acid fluorescence intensity and concentration showed good linear relationship with the correlation coefficient of 0.9963.The detection limit was 0.081 μg/mL,and the relative standard deviation was 0.54%.The method was sensitive,stable,and successfully used for the determination of chlorogenic acid in Honeysuckle and Eucommia Bark.The results also showed that chlorogenic acid in Honeysuckle and Eucommia Bark have good antioxidant activity.