槲皮素对HeLa细胞辐射敏感性影响及其机理

Effect of Quercetin on radio-sensitivity of HeLa cells

为探讨药物槲皮素对HeLa宫颈癌细胞的辐射敏感性影响及其机理,使用不同浓度的槲皮素和不同照射剂量处理HeLa细胞,采用克隆形成法测定细胞存活率,应用流式细胞术测定细胞DNA双链断裂,以及测定槲皮素与电离辐射联合作用对HeLa细胞的周期影响。结果显示,不同槲皮素浓度处理组的HeLa细胞在不同照射剂量组间的存活率比较有统计学差异(p<0.01),表明槲皮素对HeLa细胞具有明显的辐射增敏作用。槲皮素作用照射后HeLa细胞未修复的DSBs量增加,而没有减少照射后细胞G2/M期阻滞,由此可以推断槲皮素对HeLa细胞辐射敏感性影响机理可能与抑制DNA损伤的修复有关,但与细胞周期分布及G2/M期阻滞无关。

In order to investigate the mechanism of Quercetin on radio-sensitivity of human Uterine Cervix Cancer HeLa cells,HeLa cells were cultured in different concentrations of Quercetin and different doses of irradiation.The clonogenic assay was used to observe the cell servival rate.The repair of DNA double-strand breaks and effect of Quercetin combination of radiation on the cell cycle were detected by flow cytometry.The results show that the ra-dio-sensitivity of Quercetin on HeLa cells was obvious and the unrepaired DSBs after irradiation increased,but did not decrease G2 / M cell cycle arrest.From this it can be inferred that the effect on HeLa cell radio-sensitivity may be related to the inhibition of the repair of DNA double-strand breaks induced by Quercetin,but it dose not reveal a sig-nificant relation with the cell cycle and G2/M arrest.