四种常见纳米材料对骨代谢的影响

EFFECTS OF SINGLE-WALLED CARBON NANOTUBES,MULTIWALLED CARBON NANOTUBES,QUANTUM DOTS AND SILVER NANOPARTICLES ON BONE METABOLISM

探讨4种常见纳米材料(单壁碳纳米管,多壁碳纳米管,纳米银,量子点)对小鼠骨代谢的影响.选取8周龄健康昆明小鼠给予不同剂量的上述4种纳米材料,各纳米材料分别设高、中、低剂量组,溶剂对照、离子对照组,以及阳性对照组,每周3次进行腹腔注射.用药6周后检测骨破坏情况、骨生物力学改变以及血清中钙的含量.结果显示,4种纳米材料与其相应溶剂对照组相比,多壁碳纳米管溶骨发生率显著增高,高、中剂量组骨灰干重比及3个剂量组生物力学指标有显著性差异,高剂量组血清钙显著升高;单壁碳纳米管溶骨发生率上升,高、中剂量组骨灰干重比及生物力学指标降低,各组血清钙水平无显著性差异;量子点各剂量组溶骨发生率无差异,高、中剂量组骨灰干重比、最大载荷、弹性载荷降低,血清钙水平无显著性差异;纳米银各剂量组溶骨发生率未增加,灰干重比、生物力学各指标及血清钙水平无统计学差异.多壁碳纳米管长期暴露可引起小鼠溶骨性破坏、骨量下降、骨强度减低;单壁碳纳米管与量子点对骨代谢的影响不显著,纳米银不会引起骨代谢改变.

To observe the effects of four kinds of common nanoparticles（single-walled carbon nanotubes（SWNTs）,multiwalled carbon nanotubes（MWNTs）,quantum dots（QDs） and silver nanoparticles（nAg）on bone metabolism in mice.8-week-old Kunming mice were treated for 6 weeks with the nanoparticles at 3 doses or vehicle by peritoneal injection three times per week.The whole body X-ray,biomechanical parameters and serum Ca levels were measured to evaluate bone metabolism of the mice.Compared to their vehicle controls,osteolysis incidence of tibia was significantly increased in MWNTs,and there is clear differences between MWNTs and vehicle control in the bone ash ratio and the bone biomechanical properties,besides serum Ca levels was increased in high dose;osteolysis incidence was significantly increased in SWNTs,and bone ash ratio and the bone biomechanical properties were significantly decreased in high dose and middle dose,while there was no significantly differences in serum Ca levels;there wasn′t clear differences in osteolysis incidence and serum Ca levels between QDs and vehicle control,however,statistical significances were found in bone ash ratio,maximum load and elastic load of high dose and middle dose;nonsignificant results was found in different doses of nAg.Chronic exposure to multiwalled carbon nanotubes can promote osteolysis,reduce bone mineral density and change the bone biomechanical properties;single-walled carbon nanotubes and quantum dots probably influence the bone metabolism;silver nanoparticles don′t influence the bone metabolism.