紫外分光光度法测定新斯的明片中新斯的明的含量

Content Determination of Neostigmine Tablets by UV Spectrophotometry

目的:建立测定新斯的明片中新斯的明含量的方法。方法:采用紫外分光光度法。以水为溶剂,在260nm波长下测定新斯的明的含量。结果:新斯的明检测浓度在80～560μg·mL-1范围内与吸光度线性关系良好(r=0.9999);平均回收率为100.7%,RSD=0.65%(n=9)。结论:该法操作简便、精密度好,结果可靠,可用于新斯的明片的含量测定。

OBJECTIVE：To establish the method for the content determination of neostigmine tablets.METHODS：The contents of neostigmine were determined at 260 nm with water as the solvent by UV spectrophotometry.RESULTS：The calibration curve of neostigmine was linear when its concentration range was from 80 to 560 μg·mL-1（r=0.999 9）.The average recovery rate was 100.7%（RSD=0.65%,n=9）.CONCLUSION：The method is easy to operate,highly accurate,reliable in results and can be used for the content determination of neostigmine tablets.