6株芽孢杆菌的生物学特性比较研究

Comparative Study on Biological Characteristics of Six Bacillus Strains

以实验室分离保存的6株芽孢杆菌为材料,对其主要生物学特性进行了研究,包括产酶活力,对NaCl、牛胆盐、人工胃液和人工肠液的耐受性及对抗生素的敏感性。结果显示,枯草芽孢杆菌1、凝结芽孢杆菌和蜡样芽孢杆菌均能产生较高活性的中性蛋白酶和淀粉酶;2株枯草芽孢杆菌和凝结芽孢杆菌能耐较高浓度的NaCl;枯草芽孢杆菌2、凝结、纳豆和蜡样芽孢杆菌对牛胆盐的耐受性较强;除地衣芽孢杆菌外,其他5株芽孢杆菌对胃液和肠液的耐受性较好;6株芽孢杆菌均对常用饲用抗生素敏感。结果表明,枯草1、凝结、蜡样芽孢杆菌是优良的候选益生菌菌株。

The main biological characteristics of six Bacillus strains isolated were studied,including the activity of enzyme-producing,the endurance to NaCl,bile salt and artificial gastric juice as well as artificial intestinal fluid and the sensitivity to antibiotics.The results showed that,Bacillus subtilis strain 1,B.coagulans and B.cereus had the capability of producing high yield of protease and amylase.Two strains of B.subtilis and B.coagulans showed well resistance to high concentration of NaCl.B.subtilis strain 2,B.coagulans and B.natto as well as B.cereus had relatively strong tolerance for bovine bile salt.Except B.licheniformis,the other five strains of Bacillus could resist the artificial gastric and intestinal fluid well.Six strains of Bacillus were sensitive to common forage antibiotics.These results showed that B.subtilis 1,B.coagulans and B.cereus were excellent probiotics.