促性腺激素受体在雌性水牛生殖器官的表达定位研究

Immunohistochemical Localization of Gonadotropin Receptor on the Genital in Female Buffalo

为研究促性腺激素受体(FSHR、LHR)在广西雌性水牛生殖器官中的分布情况,运用免疫组化SABC法对处于不同发情周期(卵泡期、黄体期)成年水牛的卵巢、子宫、输卵管中FSHR、LHR分别进行染色定位。结果表明,FSHR/LHR阳性细胞在卵巢主要见于卵巢内膜细胞及卵泡颗粒细胞;子宫主要见于子宫内膜上皮细胞和腺体细胞;输卵管主要见于柱状上皮纤毛细胞。其中,随着发情周期不同,FSHR、LHR的表达量也有所差异,卵巢中卵泡期FSHR、LHR的表达量均高于黄体期;子宫中FSHR的表达量卵泡期高于黄体期,LHR的表达量黄体期高于卵泡期;而输卵管并没有显著差异。

To study the distribution and localization of the gonadotropin receptor（FSHR,LHR） in the position of the female buffalo genital.The localization of FSHR and LHR in adult buffalo ovary,uterus and oviduct during the follicular phase and the luteal phase were determined by immunohistochemical SABC techniques.The results showed that FSHR/LHR immunoreactive substance was mainly distributed in the thecal cells and granulose cells in the ovary,the endometrial epithelial cells,glandular cells in uterus,the ciliated columnar epithelial cells in the oviduct.The expression of FSHR and LHR was dependent on the stage of the estrus cycle,with the estrus in which different,FSHR,LHR expression were also different,the expression of FSHR,LHR in ovarian are higher during the follicle phase than those during the luteal phase.the expression of FSHR in uterus was higher during the follicle phase than during the luteal phase,the expression of LHR in uterus was higher during the luteal phase than during the follicular phase.The expression of oviduct was no significant differences.