冷应激后民猪肝素结合性表皮生长因子基因表达研究

Study on Expression of Min Pig Hepafin-binding Epidermal Growth Factor-like Gene in Cold Stress

肝素结合性表皮生长因子(hepafin-binding epidermal growth factor-like,HB-EGF)是表皮生长因子(epidermal growth factor,EGF)家族的成员之一。本研究采用Real-time PCR分析了在民猪冷诱导后该基因在骨骼肌内的表达变化情况。结果表明,低温组(-20℃)的HB-EGF基因的表达水平与常温组(10℃)相比出现了极显著下降(P<0.01)。据此推测,冷应激抑制了HB-EGF基因的表达,也间接抑制了民猪的正常生长。

Hepafin-binding epidermal growth factor-like（HB-EGF） is one of the epidermal growth facto family.In this study,Real-time PCR method was used to detect and analysis the expression of HB-EGF gene in Min pig skeletal muscle after cold-induced.The results showed that the low-temperature group（-20 ℃） of HB-EGF gene expression levels compared with the normothermia group（10 ℃） appeared very significantly decreased（P0.01）.As above,cold stress inhibits the expression of HB-EGF gene,but also indirectly inhibit the normal growth of Min pig.