摘要
通过不同方式处理猪外周血淋巴细胞,对不同保存时间的淋巴细胞进行流式细胞术检测,探讨其对T淋巴细胞亚群中CD3+、CD4+、CD8+亚群的影响。采用肝素钠、EDTA、枸橼酸钠3种抗凝剂抗凝猪外周静脉血,分别在第1、2、4、6、8、10天用流式细胞仪检测。结果表明,肝素钠抗凝,第1、2、4天内检测差异不显著(P>0.05),第6天检测CD4+、CD8+细胞显著减少(P<0.05),第8、10天极显著减少(P<0.01)。EDTA抗凝,第1、2、4天内检测差异不显著(P>0.05),第6天检测CD3+细胞显著减少(P<0.05),第8、10天极显著减少(P<0.01);枸橼酸钠抗凝,第1、2、4、6、8天内检测差异不显著(P>0.05),第10天检测CD3+、CD4+细胞极显著减少(P<0.01),CD8+细胞显著减少(P<0.05)。3种抗凝剂检测比较,处理后的第1、2、4、6天肝素钠抗凝管检测结果CD3+、CD4+、CD8+均高于EDTA和枸橼酸钠抗凝。本研究表明,流式细胞术检测猪外周血优选肝素钠抗凝,样本采集后4 d内检测完毕。
The experiments were designed to study the changed of T lymphocyte subset about CD3＋,CD4＋,CD8＋ which detected by triple-color flow cytometry for swine,the blood sample were collected by three different anticoagulants and detection on different days.The results showed that CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd,4th day had no significant difference,but on the 6th CD4＋,CD8＋ had significant different,on the 8th day and 10th day had extremely significant different in the heparin anticoagulant samples group.In the EDTA anticoagulant samples group CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd and 4th day had no significant difference,on the 6th day CD3＋ had significant difference,on the 8th and 10th day had extremely significant difference.In the sodium citrate anticoagulant samples group CD3＋,CD4＋,CD8＋ detected ratio on the 1st,2nd,3rd,4th,6th day and the 8th day had no significant difference,on the 10th day CD3＋,CD4＋ had extremely significant difference,and CD8＋ had significant difference.Compared three different anticoagulants,the heparin was better others,and it＇s better to detect the samples within four days.
出处
《中国畜牧兽医》
CAS
北大核心
2011年第4期101-104,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金面上项目(30972193)
