绒山羊11号染色体7个微卫星标记的连锁图谱的构建

A Linkage Map of 7 Microsatellite Markers in the 11th Chromosome of Cashmere Goats

本试验利用内蒙古白绒山羊5个家系中的632个个体,用11号染色体上的7个微卫星标记,构建绒山羊11号染色体遗传连锁图。结果表明,7个标记的等位基因数变化范围为8~14,杂合度在0.5886~0.9348之间,平均杂合度为0.8612,各标记的多态信息含量在0.7712~0.8990之间,平均多态信息含量为0.8472。构建的遗传连锁图总长度127.7 cM,其中标记ILSTS028与INRA108间距最大,为41.2 cM;ILSTS049和INRA131间距最小,为5.1 cM。

The 7 microsatellite markers in the 11th chromosome were used to construct a linkage map of Cashmere goats with a 632 samples of 5 half-sib pedigrees after paternity testing.The results showed that the allele numbers of the 7 microsatellite markers varied from 8 to 14.The lowest and the highest values of the heterozygosity were 0.5886 and 0.9348,respectively,and the average value of it was 0.8612,indicating abundant genetic diversity in Inner Monglian Cashmere goats.Moreover,the polymorphism information content（PIC） value of 7 markers was from 0.7712 to 0.8990,and the average one was 0.8472.The length of the linkage map of the 11th chromosome was 127.7 cM.The nearest genetic distance was 5.1 cM and its location was between ILSTS049 and INRA131,however,the farest one was 41.2 cM and lay in ILSTS028 and INRA108.