高糖胁迫对生长期酿酒酵母生理代谢的影响

Effect of High-glucose Addition on Metabolism of Saccharomyces cerevisiae during Logarithm Phrase

本文研究了在高糖培养基条件下酿酒酵母的生长及胞内海藻糖代谢情况。结果表明在180～400 g/L的高葡萄糖浓度下酿酒酵母均能正常生长,其中300 g/L下酿酒酵母良好生长。随着初始糖浓度增加,胞内海藻糖量迅速增加,在400 g/L的葡萄糖浓度下,胞内海藻糖积累量达48.73 mg/g(湿重)。

The intracellular trehalose metabolism and cell growth of Saccharomyces cerevisiae in high-glucose addition were studied.It was found that the yeast cells can grow well and trehalose metabolism was regulated in concentration of 180～400 g/L and it grew best in 300 g/L glocuse-containing system.The trehalose content of S.cerevisiae cells rapidly increased in response to the high-glucose medium.The accumulation of trehalose is 48.73 mg/g（wet cell weight） at the concentration of 400 g/L.