C-erbB-2、MMP-1、MMP-2、MMP-7和MTA1在哈萨克族食管癌中的表达及其临床意义

The Expression and Clinical Significance of C-erbB-2,MMP-1,MMP-2,MMP-7 and MTA1 in Esophageal Cancer of the Kazak

通过观察C-erbB-2、MMP-1、MMP-2、MMP-7和MTA1 5个基因在哈萨克族食管癌组织中的表达,探讨其侵袭转移相关因子在新疆哈萨克族食管癌组织的表达和临床意义。采用RT-PCR方法检测75例哈萨克族食管癌标本中这5个基因的mRNA表达水平,分析它们与临床病理特征的关系。结果显示,5个基因在哈萨克族食管癌组织中表达较正常组织增高,达到差异显著(P<0.05);MMP-2、MMP-7、MTA1基因的表达与淋巴结转移相关(P<0.05),MMP-1、MMP-7基因的表达与临床分期正相关(P<0.05);C-erbB-2与MTA1、MMP-2与MTA1、MMP-7与MTA1、MMP-7与MMP-1、MMP-1与MTA1基因的表达差异高度一致(P<0.01)。由此得出,这5个基因表达上调在哈萨克族食管癌的发生发展过程中协同发挥作用;MMP-2、MMP-7、MTA1可能是哈萨克族食管癌发生侵袭、转移的主导因素。

The expressions of C-erbB-2,MMP-1,MMP-2,MMP-7 and MTA1 in esophageal cancer tissue and normal tissue among the Kazak are studied and the relationship between their expressions and clinical pathological feature is investigated.The expression of C-erbB-2,MMP-1,MMP-2,MMP-7 and MTA1 gene mRNA were detected by using RT-PCR method in 75 esophageal cancer specimens of the Kazak.The results show that the positive rates of C-erbB-2,MMP-1,MMP-2,MMP-7 and MTA1 in cancer tissue are higher than that of normal tissues（P〈0.05）.While the expression mRNA level of MMP-2,MMP-7 and MTA1 is significantly related to the lymphnode metastasis,And the expression mRNA level of MMP-1,MMP-7 is significantly related to the TNM stages（P〈0.05）.Kappa analysis indicates that the expressions between C-erbB-2 and MTA1,MMP-2 and MTA1,MMP-7 and MTA1,MMP-7 and MMP-1,MMP-1 and MTA1 in the Kazak＇s esophageal cancer are positively related（κ=0.624,0.367,0.564,0.275,0.384,P〈0.01） with each other.The conclusion shows that the over expression of C-erbB-2,MMP-1,MMP-2,MMP-7 and MTA1 gene could play the important roles in the carcinogenesis of esophageal cancer in the Kazaks.Their synergistic effect could promote carcinogenesis and development of esophageal cancer.The expressions of MMP-2,MMP-7,and MTA1 might be related to the progression and metastasis of esophageal cancer among the Kazak.