肠道菌群重构Ⅱ型糖尿病大鼠胰腺细胞线粒体电镜观察

The ultrastructural changes in mitochondria of pancreatic cells from strptozotocin-induced type-2 diabetic rat remodeled intestinal flora

目的:观察肠道菌群重构Ⅱ型糖尿病大鼠胰腺细胞线粒体的超微结构变化。方法:将64只SD雄性大鼠随机分为对照组、造模组、益生组、去污组。采用髙糖髙脂喂养,亚致病剂量(30 mg/ml)链脲佐菌素腹腔一次性注射造模,在2周4、周测空腹血糖并处死大鼠取胰尾做电镜切片观察。结果:各组出现的血糖升高无显著性差异(P>0.05);益生组线粒体受损明显,去污组相对较轻。结论:肠道菌群改变后导致Ⅱ型糖尿病大鼠线粒体损伤超微病理改变程度有所不同。

Objective：To investigate the ultrastructure changes in mitochondrion of pancreatic cells from streptozotocin-induced type-2 diabetic rat remodeled intestinal flora.Methods：64 male SD rats were randomly divided into four groups,including control group,type-2-diabetic-modeling group,probiotic group and depollutional group.All groups were fed with high-fat diet and inoculated Streptozotocin（30mg/ml） by intraperitoneal injection one time except control group. Results：Fasting blood glucose increased significantly comparing with control group,while there were no significant differences among those three groups（P0.05）.Mitochondria were significantly impaired in probiotic group revealed by TEM,while the impairment in depollutional group was relatively light.Conclusion：Remodeling intestinal flora resulted in more severe ultrastructural pathology of mitochondria in pancreatic cells from streptozotocin-induced type-2 diabetic rats.