两种方法检测冻肉中的小肠结肠炎耶尔森氏菌

Study on Isolation and Identification of Yersinia enterocolitica of raw meat

目的:建立适用于本实验室快速、准确检测致病性小肠结肠炎耶尔森氏菌的方法。方法:采用常规分离培养鉴定法与多重PCR方法对49份生肉进行检测,分析比较两种方法的检测结果。结果:两种方法的检测结果相同,符合率为100%。49份样品中均检出8份检出小肠结肠炎耶尔森氏菌,3株阳性对照以及6株阴性对照也均符合检测结果。但多重PCR方法所需的检测时间远少于常规分离培养鉴定法。结论:多重PCR较传统鉴定方法省时省力,对于监测小肠结肠炎耶尔森氏菌有重要意义。

Objective：To study the methods of isolation and identification of pathogenic.Methods：Yersinia enterocolitica,isolated from samples of raw meat 49 portions,was identified by routine bacterial culture and multiplex PCR respectively.Results：The isolation and identification results of the two identification methods were consistent.Eight strains of Yersinia enterocolitica were isolated and identified from raw meat of 49 portions.Conclusion：Being time-and labor-saving,the multiplex method has superiority over conventional identification methods and it can be effectively employed in surveillance of Yersinia enterocolitica.