摘要
目的:建立适用于本实验室快速、准确检测致病性小肠结肠炎耶尔森氏菌的方法。方法:采用常规分离培养鉴定法与多重PCR方法对49份生肉进行检测,分析比较两种方法的检测结果。结果:两种方法的检测结果相同,符合率为100%。49份样品中均检出8份检出小肠结肠炎耶尔森氏菌,3株阳性对照以及6株阴性对照也均符合检测结果。但多重PCR方法所需的检测时间远少于常规分离培养鉴定法。结论:多重PCR较传统鉴定方法省时省力,对于监测小肠结肠炎耶尔森氏菌有重要意义。
Objective:To study the methods of isolation and identification of pathogenic.Methods:Yersinia enterocolitica,isolated from samples of raw meat 49 portions,was identified by routine bacterial culture and multiplex PCR respectively.Results:The isolation and identification results of the two identification methods were consistent.Eight strains of Yersinia enterocolitica were isolated and identified from raw meat of 49 portions.Conclusion:Being time-and labor-saving,the multiplex method has superiority over conventional identification methods and it can be effectively employed in surveillance of Yersinia enterocolitica.
出处
《中国卫生检验杂志》
CAS
2011年第3期628-629,632,共3页
Chinese Journal of Health Laboratory Technology
基金
广州市卫生局医药卫生科技资助项目(2006-Zdi-11)