脊髓灰质炎病毒与大肠杆菌热抗力的比较及有机物对热抗力影响的研究

Comparison of thermo-resistance differences between Poliovirus and Escherichia coli and influence of organic substance on thermo-resistance efficacy

目的:比较脊髓灰质炎病毒和大肠杆菌的热抗力及有机物对热抗力的影响。方法:采用干热和湿热条件下不同温度和不同时间灭活的方法进行试验。结果:湿热条件下50℃、60℃和70℃分别作用20 min、10 min和5 min,干热条件下60℃、80℃和100℃分别作用20 min、10 min和5 min可使脊髓灰质炎病毒全部灭活。湿热条件下60℃、70℃和80℃分别作用90 min、10 min和3 min,干热条件下100℃、120℃和140℃分别作用60 min、15 min和10 min可使大肠杆菌全部灭活。湿热条件下有机物对大肠杆菌的热抗力无影响,干热低温条件下TSB对大肠杆菌的热抗力有一定影响。结论:大肠杆菌的热抗力明显强于脊髓灰质炎病毒,有机物TSB对大肠杆菌的热抗力有一定影响。

Objective：To compare the thermo-resistance between Poliovirus and Escherichia coli and the influence of organic substance on efficacy of thermo-resistance.Methods：In dry heat and moist heat,to use different times,and different temperatures,testing the thermo-resistance of Poliovirus and Escherichia coli, and the organic substances on efficacy of thermo-resistance.Results：Poliovirus could all inactivated in moist heat condition,heating in 50℃ 20 min,60℃10 min or 70℃ 5 min;and in dry heat condition,heating in 60℃ 20 min,80℃10 min,or 100℃ 5 min. E.coli could all inactivated in moist heat condition,heating in 60℃ 90 min,70℃10 min or 80℃ 3 min;and in dry heat condition,heating in 100℃ 60 min,120℃15 min or 140℃ 10 min. The organic substances did not influence the E.coli thermo-resistance in moist heat condition.Interestingly,TSB influenced the E.coli thermo-resistance in dry heat condition with low temperature.Conclusion：E.coli had higher thermo-resistance than Poliovirus.The TSB had some influence on E.coli thermo-resistance.