生殖道人乳头状瘤病毒基因型检测及临床分析

Clinical analysis of human papilloma virus genotype with genital

目的:讨论女性生殖道人乳头状瘤(HPV)感染的类型及临床意义。方法:采用反向寡核苷酸探针杂交技术(PCR-RDB)对1524例女性生殖道标本进行HPV分型检测,分析各型的感染率以及感染病毒与年龄段关系及HPV分型在宫颈病变中的意义。结果:1524分标本共检出HPV阳性279例总体阳性率为18.31%,PCR-RDB技术所涉及到的23种HPV基因型均被检出,其中高危型以16、185、85、2型为主,低危型以11、6型为主,多型混合感染多发生在15岁～25岁和46岁～55岁,随着宫颈糜烂程度的增加多重感染数量也增加。结论:女性生殖道HPV感染高危型以16、18型为主,低危以6、11为主;在预防和治疗宫颈病变时应重视HPV亚型的混合感染。

Objective：To discuss the type of human papillomavirus infection of female genital tract and its clinical significance.Methods：Using reverse oligonucleotide microarray probes hybridization techonology to typing test 1524 sepecimens of female genital tract,to analyze infection rates of various types,relationship between infection virus and age and the significance of HPV typing in cervical lesions.Results：There＇re 279 cases infected from HPV in 1524 sepecimens with 18.31% positive rate,23 kinds of HPV genotypes involve in PCR-RDB techonology were all detected,with 16,18,58,52 taking great important part in high-risk type and 11,6 in low-risk type.Types of mixde infection mainly ocurs in age of 15 to 25 and 46 to 55,and the number of multiplicity of infection increases with the degree of cervical erosion.Conclusion：In human papillomavirus infection of female genital tract,16,18 take important part in high-risk type and 11,6 in low-risk type;Mixed infection of HPV subtypes should be foucs in the prevention and treatment of cervical lesions.