小微粒色谱柱-高效液相色谱紫外检测器法测定花生中黄曲霉毒素

Small particle column-high performance liquid chromatography UV detection method for the determination of aflatoxins in peanuts

目的:建立用小微粒色谱柱-高效液相色谱法来测定花生中黄曲霉毒素的方法。方法:样品打碎均匀后,用Bond Elut PH柱萃取,以V水∶V甲醇∶V乙腈=50∶40∶10为流动相,经小微粒色谱柱分离,紫外检测器检测。结果:四种黄曲霉毒素G2、G1、B2、B1得到很好的分离.检测限分别为:0.5μg/kg0、.8μg/kg、0.2μg/kg、0.4μg/kg。花生样品中黄曲霉毒素G2、G1、B2、B1在添加浓度为0.3μg/kg～3μg/kg的范围内,平均回收率在:82%～96%。相对标准偏差在1.2%～3.3%。结论:该法能在短时间内使花生中黄曲霉毒素G2、G1、B2、B1得到很好的分离,准确,比国标法快捷,值得推广。

Objective：To establish a column with small particles-high performance liquid chromatography to determine aflatoxin in peanuts.Methods：The sample was broken even after extraction with Bond Elut PH columns,with V（H2O）∶VCH3OH）∶V（CH3CN）=50∶40∶10 as mobile phase,separated by a small particle column,UV detector.Results：Four aflatoxin G2,G1,B2,B1 are well separated.Detection limits were：0.5 μg/kg,0.8 μg/kg,0.2 μg/kg,0.4 μg/kg.Samples of peanut aflatoxin G2,G1,B2,B1 concentration was 0.3 μg/kg～3 μg/kg in the range,the average recovery rate：82%～96%.The relative standard deviation of 1.2%～3.3%.Conclusion：This method can in a short time make peanut aflatoxin G2,G1,B2,B1 are well separated,accurate and efficient than the national standard,worthy of promotion.