引起黄蜀葵黄脉症状双生病毒的分子鉴定

Molecular Identification of Geminivirus Inducing Vein Yellowing in Abelmoschus manihot

目的:采用分子生物学方法鉴定从广西药用植物园科研基地种植的表现曲叶、黄脉的黄蜀葵上得到病毒分离物H。方法:在电镜下观察病毒粒子的形状,并采用双生病毒的简并引物PA和PB对病样进行PCR检测。结果:从分离物H中扩增得到特异片段有同源性的均属双生病毒科菜豆花叶病毒属病毒成员,其中与一点红黄脉病毒的分离物相似性最高,在核苷酸水平上为95%。结论:引起黄蜀葵黄脉症状的病害存在双生病毒侵染,该病害可能是由一点红黄脉病毒引起。

Objective：The virus isolate H was identified by molecular biology,it was collected from Abelmoschus manihot plant showing leaf curl,yellow vein symptoms in Guangxi Botanical Garden of Medicinal Plant.Methods：The virus isolate H was observed in electron micrograph,and conformed detected by PCR using universal primer pair for the genus Geminivirus.Results：The results indicated that all sequences homologous to the specific fragment belonged to the genus Begomovirus of the family Geminiviridae.There was the highest similarity shared 95% homology at nucleotide between the specific fragment and DNA-A of Emilia yellow vein virus isolates.Conclusion：These findings suggested that there was geminiviridea in Abelmoschus manihot,and the disease probably caused by Emilia yellow vein virus.