川贝母细胞团块悬浮培养生产生物碱的研究

Study on the Production of Alkaloid by Cell Mass Suspension Culture of Fritillaria cirrhosa

目的:建立川贝母细胞团块悬浮培养体系,筛选出快速获取细胞团块增殖的最佳培养条件和激素配比。方法:以MS培养基为基本培养基,比较了接种量、激素配比和生长调节物质等对川贝母细胞团块悬浮培养的影响,并比较了细胞团块在不同培养条件下的生长情况,测定了增殖细胞团块中总生物碱含量。结果与结论:用川贝母细胞团块进行悬浮培养,其生长速度明显快于固体培养;细胞团块中总生物碱含量均高于市售川贝母和野生川贝母鳞茎;川贝母细胞团块悬浮培养最适的接种量为30 g/L,最佳培养基为MS+6-BA 2.0 mg/L+NAA 0.2 mg/L。

Objective：Set up Fritillaria cirrhosa cell mass suspension culture system to rapidly screen the best culture conditions for cell mass proliferation and hormone combination.Methods：Using MS medium as the basic medium,the impact of inoculum size,hormone combination,growth regulators for Fritillaria cirrhosa cell mass suspension culture were compared,and also the growth of cell mass at different culture conditions was compared,and the total alkaloids content in proliferative cell mass was measured.Results and Conclusion： Fritillaria cirrhosa grow significantly faster in cell mass suspension culture than in the solid culture.The total alkaloid content in cell mass is higher than commercial and wild bulb of Fritillaria cirrhosa.The optimal inoculum size for cell mass suspension culture is 30 g/L and the optimal culture media is MS＋6-BA 2.0 mg/L＋NAA 0.2 mg/L.