戈米辛J和异型南五味子丁素对大鼠胸主动脉的作用

Effects of Gomisin J and Heteroclitind on Rat Thoracic Aorta

目的研究由南五味子属药用植物分离的戈米辛Ｊ（ｇｏｍｉｓｉｎＪ，ＧＪ）和异型南五味子丁素（ｈｅｔｅｒｏｃｌｉｔｉｎＤ，ＨＤ）对血管平滑肌的作用。方法采用离体大鼠胸主动脉标本，观察他们对高钾去极化收缩和对ＣａＣｌ２、ＮＡ量效曲线的影响。结果ＧＪ和ＨＤ能抑制ＫＣｌ所致的收缩，其ＩＣ５０（９５％可信限）分别为３．８（２．４～５．９）和７．５（１．４～３９）μｍｏｌ／Ｌ；ＧＪ和ＨＤ能使ＣａＣｌ２量效曲线右移，最大效应降低，其ｐＤ′２值分别为５．１３±０．０７和４．７７±０．１８；ＧＪ和ＨＤ也可使ＮＡ量效曲线右移和最大效应降低，其ｐＤ′２值分别为３．７２±０．２３和３．３１±０．２７。结论ＧＪ和ＨＤ能抑制ＫＣｌ、ＣａＣｌ２和ＮＡ产生的血管收缩，而且对ＣａＣｌ２的致缩作用强于对ＮＡ的收缩作用，提示他们具有钙拮抗活性。

Purpose To investigate the effects of gomisin J(GJ) and heteroclitin D (HD) from Kadsura medicinal plants on vascular smooth muscle. Methods Isolated rat aortic ring preparations were used to observe the effects of GJ and HD on KCl,CaCl 2 and NAinduced contractions. Results GJ and HD inhibited KCl induced contractions with IC 50 (95%CL)3.8(2.4-5.9)μmol/L and 7.5(1.4-39) μmol/L,respectively.Inhibitory effects of GJ and HD were more potent on CaCl 2 induced contractions than on NA induced contractions.The pD′ 2 values on CaCl 2 induced contractions were 5.13±0.07 and 4.77±0.18,respectively;the pD′ 2 values on NA induced contractions were 3.72±0.23 and 3.31±0.27. Conclusions GJ and HD can inhibit KCl,CaCl 2 or NA induced contractions,suggesting that they may act as a calcium channel blocker.