弥漫性脑损伤大鼠皮质和海马中BrdU和Nestin蛋白的表达

Expression of BrdU and Nestin protein in cortex and hippocampus of rats with diffuse brain injury

目的:观察大鼠弥漫性脑损伤(DBI)后不同时间皮质和海马中5-溴-2-脱氧尿苷(BrdU)和巢蛋白(Nes-tin)蛋白的变化,探讨其与脑损伤时间的关系。方法:成年健康SD大鼠36只,随机分为对照组(6只)和实验组(30只)。实验组大鼠制作DBI模型后用免疫组织化学方法观察脑损伤后不同时间(1、3、7、14和28d,n=6)皮质和海马中BrdU和Nestin蛋白的表达。结果:与对照组相比,实验组大鼠DBI后不同时间脑皮质、海马中的BrdU及Nes-tin阳性细胞均增加(BrdU:F皮质=141.447,F海马=48.361,P均<0.001;Nestin:F皮质=94.159,F海马=98.183,P均<0.001)。伤后1d,皮质和海马内就有BrdU和Nestin阳性细胞的大量表达;皮质内BrdU阳性细胞的表达于3d达到高峰,Nestin阳性细胞的表达于7d达到高峰,均持续至第14天后开始下降;海马内BrdU和Nestin阳性细胞的表达于第7天达到高峰,其后逐渐下降。结论:BrdU和Nestin蛋白表达所呈现出的时序性变化规律,可望用于DBI损伤时间的推断。

Aim：To observe the alteration of BrdU and Nestin positiveness cells in the diffuse brain injury（DBI） rat and its relation to the injury intervals.Methods：A total of 36 healthy adult SD rats were randomly divided into control and experimental groups.Based on Marmarou＇s model of DBI and took the samples at 1 d,3 d,7 d,14 d,and 28 d after brain injury.The expressions of BrdU and Nestin were observed by immunohistochemical staining in combination with imaging analysis.Results：Compared with the control group,at 1 day post-trauma,a significant increase in BrdU and Nestin postive cells was observed in the cortex and hippocampus tissue（BrdU：FCortex=141.447,FHippocampus=48.361,P0.001;Nestin：FCortex=94.159,FHippocampus=98.18,P0.001）.The expression level of BrdU peaked at 3 days after injury and Nestin peaked at 7 days after injury in cortex tissue,then they decreased gradully at 14 days after injury.The expression level of BrdU and Nestin peaked at 7 days in hippocampus tissue,then they decreased gradually.Conclusion：Time-order expression of BrdU and Nestin could be used to estimate the time of brain injury in forensic pathology.