颈动脉粥样硬化斑块组织中ABCA1和MMP-9的表达

Expression of ABCA1 and MMP-9 in carotid atherosclerotic plaques tissue

目的:探讨三磷酸腺苷结合盒运转体A1(ABCA1)及基质金属蛋白酶-9在人颈动脉粥样硬化斑块中的表达变化及其与斑块形成的关系。方法:收集人颈动脉粥样硬化斑块标本36例和16例肠系膜动脉标本(对照组,B组),根据病理Ⅰ、Ⅱ和Ⅲ分级相应将颈动脉粥样硬化斑块标本分为A1(n=5)、A2(n=8)和A3(n=23)组。采用RT-PCR技术测定ABCA1和MMP-9mRNA的表达,Westernblot检测ABCA1和MMP-9的蛋白的表达。结果:4组间ABCA1和MMP-9mRNA的表达比较,差异有统计学意义(F=863.954和717.381,P<0.001)。A组较B组升高,A3组较A2组升高(P<0.05)。4组间ABCA1和MMP-9蛋白表达的比较,差异有统计学意义(F=360.612和428.910,P<0.001)。ABCA1蛋白的表达A组较B组降低,A3组较A2组降低;MMP-9蛋白的表达A组较B组升高,A3组较A2组升高(P<0.05)。结论:ABCA1和MMP-9可能是进展性动脉粥样硬化损害的关键因素。

Aim：To investigate the expressions of ATP-binding cassette transporter A1（ABCA1） and matrix metalloproteinase-9（MMP-9） in human carotid atherosclerotic plaques.Methods：36 cases of human carotid atherosclerotic plaque specimens and 16 specimens of patients with mesenteric artery（control group） were collected after operation.The carotid plaque specimens were divided into group A1（grade Ⅰ）,group A2（gradeⅡ）,and group A3（grade Ⅲ） according to their histological grade.Results：transcriptase polymerase chain reaction（RT-PCR） was used to detect the expression of ABCA1 and MMP-9 mRNA,And Western blot technique was used to detect the expression of ABCA1 and MMP-9 proteins.The expressions of ABCA1 and MMP-9 mRNA among 4 groups were different,there were obvious differences（F=863.954 and 717.381,P0.001）.The expressions of ABCA1 and MMP-9 mRNA were higher in group A than that in group B and higher in group A3 than that in group A1.The expressions of ABCA1 and MMP-9 protein among 4 groups were different,there were obvious differences（F=360.612 and 428.910,P0.001）.The expressions of ABCA1 protein were lower in group A than that in group B and lower in group A3 than that in group A1.The expressions of MMP-9 protein were higher in group A than that in group B and higher in group A3 than that in group A1.Conclusion：The changes of the expression levels of ABCA1 and MMP-9 might play a key role in the pathogenesis of atherosclerosis.