子痫前期患者胎盘组织中晚期糖基化终产物受体和P-选择素的表达

Expression of receptor of advanced glycation end products and P-selectin in placenta of preeclampsia patients

目的:探讨晚期糖基化终产物受体(RAGE)和P-选择素(P-selectin)在子痫前期(PE)发生发展中的作用。方法:采用免疫组织化学SP法和RT-PCR法检测30例正常孕妇(对照组)和40例PE患者(轻度20例、重度20例)胎盘组织中RAGE和P-selectin蛋白及mRNA的表达情况。结果:对照组与PE组胎盘组织内均有RAGE和P-selectin阳性表达,RAGE主要表达于滋养细胞,血管内皮细胞中也有少量表达;P-selectin主要表达于合体滋养细胞、血管内皮细胞及间质细胞。3组RAGE和P-selectin蛋白(F=250.152和274.044,P均<0.001)及mRNA(F=624.550和708.351,P均<0.001)比较,差异均有统计学意义。轻、重度PE组RAGE、P-selectin蛋白和mRNA表达高于对照组(P均<0.05),重度PE组高于轻度PE组(P均<0.05);PE患者胎盘组织中2种蛋白及mRNA表达均呈正相关(r=0.814和0.796,P均<0.001)。结论:RAGE和P-selectin表达升高可能与PE的发生发展有关,二者在PE的发病中有协同作用。

Aim：To explore the role of receptor of advanced glycation end products（RAGE） and P-selectin in the development of preeclampsia（PE）.Methods：SP method and RT-PCR analysis were used to detect the expression of RAGE and P-selectin protein and mRNA in placenta tissue from 40 cases of PE（20 cases of mild PE,20 cases of severe PE） and 30 cases of normal term pregnant.Results：All groups showed RAGE and P-selectin positive pigmentation in the placenta.RAGE protein was mainly expressed in trophoblast cells,but there was a small amount in the vascular endothelial cells.P-selectin was mainly expressed in syncytiotrophoblast cells,vascular endothelial cells,and interstitial cells.There were significant differences among three groups on the expression of RAGE and P-selectin protein（F=250.152 and 274.044,P0.001） and mRNA（F=624.550 and 708.351,P0.001）.Expression of RAGE and P-selectin mRNA was significantly higher in placenta tissue of PE group compared with that of control group（P0.05）,the levels of severe PE group were higher than those of mild PE group（P0.05）,and there was a positive correlation between them of protein and mRNA expression（r=0.814 and 0.796,P0.001）.Conclusion：The high levels of RAGE and P-selectin in placenta tiusse in PE may be associated with the occurrence and development of PE,and both of them have synergies in the development of PE.