非霍奇金淋巴瘤组织中p73基因印迹状态及甲基化

Methylation and imprinting status of p73 gene in tissue of Non-Hodgkin's lymphoma

目的:探讨p73基因启动子区甲基化及基因印迹状态与非霍奇金淋巴瘤的关系。方法:取淋巴结活检新鲜标本40例,其中非霍奇金淋巴瘤20例(B细胞淋巴瘤16例,T细胞淋巴瘤4例),淋巴结反应性增生20例。采用逆转录聚合酶链反应-限制性片段长度多态性(RT-PCR-RFLP)的方法及甲基化特异性聚合酶链反应(MS-PCR)的方法分别检测上述组织中p73基因的印迹状态及启动子区域CpG岛的甲基化状态。结果:20例非霍奇金淋巴瘤p73基因杂合子表型8例,其中p73基因单等位基因表型7例;20例淋巴结反应性增生中p73基因杂合子表型6例,其中p73基因单等位基因表型5例,2组间等位基因表型比较差异无统计学意义(P>0.999)。20例非霍奇金淋巴瘤组织中p73基因启动子区甲基化频率为35.0%,而20例淋巴结反应性增生中均无p73基因启动子区甲基化(P=0.008)。结论:p73基因异常甲基化可能与非霍奇金淋巴瘤的发生有关。

Aim：To study the relationship between the methylation and imprinting status of p73 gene promoter region and non-Hodgkin＇s lymphoma.Methods：A total of 40 lymph node biopsy specimens,including 20 specimens of non-Hodgkin＇s lymphoma（16 specimens of B-cell lymphoma,4 specimens of T-cell lymphoma） and 20 specimens of reactive hyperplasia of lymph node.We detected the gene imprinting and promoter region CpG Island methylation status of p73 gene in these tissues by reversed transcript PCR with restriction fragment length polymorphism（RT-PCR-RFLP）method and methylation-specific polymerase chain reaction（MS-PCR）method,respectively.Results：There were eight cases of heterozygous phenotype of p73 gene in 20 cases of non-Hodgkin＇s lymphoma,in which the number of cases of p73 gene monoallelic phenotype was seven;There were six cases of heterozygous phenotype of p73 gene in 20 cases of reactive hyperplasia of lymph node,in which the number of cases of p73 gene monoallelic phenotype was five.p73 allelotype between the two groups had no statistical significance（P0.999）.The methylation rate of p73 gene in non-Hodgkin＇s lymphoma was 35.0%,while the rate of control group was 0（P=0.008）.Conclusion：Abnormal methylation of p73 gene may be related to non-Hodgkin＇s lymphoma.