大鼠脑损伤后非损伤区域缺氧诱导因子与乳酸的变化研究

The Change of Hypoxia Inducible Factor and Lactic Acid in Non-damaged Regions After Brain Injury

目的:研究大鼠脑损伤后非损伤区域缺氧诱导因子(hypoxia-inducible factor-1α,HIF-1α)与乳酸的表达变化。方法:取雄性SD大鼠36只,体重200-300g,参照统计学随机数字表将大鼠随机平均分为正常对照组(6只)、假手术组(6只)、造模组(24只),3组,造模组分四个时间点12h、72h、1w、2w处死动物(每时间点6只)。使用立体定位仪和液压打击装置,靶向打击大脑中动脉,造大鼠脑外伤模型。采用免疫组织化学法检测脑外伤后不同时间点损伤临近区域脑组织中HIF-1α蛋白表达及乳酸含量的变化。结果:正常组和假手术组脑组织神经细胞HIF-1α表达和乳酸含量无明显变化,而模型组损伤临近区域HIF-1α的表达及乳酸含量的变化规律基本一致,12 h时增多,72h时达到高峰,1w表达下降至2w时恢复正常。造模组12h、72h、1w3个亚组与正常对照组比较差异具有统计学意义p<0.01,造模组2w亚组与正常对照组比较差异无统计学意义p>0.01。结论:脑外伤后非损伤区域也有缺血、缺氧的改变,可能与脑外伤后的脑萎缩有相关性。

Objective：To study the expression of hypoxia induced factor and Lactate in non damage area of traumatic brain injuried rat.Methods：36 male SD rats,weight 200-300g,by statistical random number table,were randomly divided into normal control group（6）,sham operation group（6）,model group（24）,rat of the third group were sacrificed in four time points 12h,72h,1w,2w.The third group is injuried with stereotaxic apparatus and hydraulic blow device,targeted blow middle cerebral artery.At different time points examined HIF1-α and Lactate in the area surrounding injuried.Results：Expression of HIF1-α and lactate in normal group and sham operation group showed no significant change,contrast with the third group,increased in 12h,reached peak in 72h,decreased in 1w and resume to normal in 2w.Groups of 12h,72h and 1w compared with the control group was statistically significant difference p0.01.Conclusion：Non-injury regions after traumatic brain injury have ischemia,hypoxia changes and may be related to brain atrophy after traumatic brain injury are related.