摘要
选择合适的蛋白表达系统是外源基因能否成功表达的关键。毕赤酵母(Pichia pastoris)蛋白表达系统是近些年来发展起来的一种真核表达系统,与其他表达系统相比,该系统所具有的诸多优势使其研究价值和应用价值越来越广泛,已经成功表达了多种蛋白质。简要综述其特点、表达宿主菌、表达载体以及其元件、外源蛋白的表达及其影响因素等方面的基础研究和最新进展。
The selection of appropriate protein expression system is the key to express heterologous gene successfully.Pichia pastoris protein expression system has developed a eukaryotic expression system in recent years.The value of its research and application is very extensive for its many advantages compared with other expression systems.At present,Pichia pastoris protein expression system has successfully express a variety of proteins.In this paper,we briefly reviewed the P.pastoris protein expression system on its characteristics,host strains,expression vectors and their elements,the expression of foreign proteins and their influencing factors in the areas of basic researches and the newest advances.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第4期46-51,共6页
Biotechnology Bulletin
基金
福建省科技厅资助项目(2009N0032)
福建省教育厅资助项目(JA08041)
关键词
毕赤酵母
表达系统
外源蛋白
载体
研究进展
Pichia pastoris Expression system Foreign proteins Vector Research advance