摘要
为建立高效的农杆菌介导的紫花苜蓿的遗传转化体系,对影响转化体系的若干因素进行了研究。结果表明,最适宜的条件分别为抗菌素为350 mg/L的羧苄青霉素(Carb);卡那霉素(Kan)筛选的浓度为60 mg/L;基因型为WL-323;外植体为下胚轴;农杆菌菌液浓度OD600值为0.4-0.6;侵染时间10 min;乙酰丁香酮(AS)的浓度为10 mg/L。
In order to establish high-efficiency alfalfa genetic transformation system,the influence factors of Agrobacterium-mediated transformation system were studied.The results showed that the most suitable conditions for Carbenicillin（Carb）was 35 mg/L;Kanamycin（Kan）was 60 mg/L;the genotypes was WL-323;the explantwas hypocotyl;OD600 of bacterial concentration value for 0.4-0.6;the infection time was 10 min;Acetosyringone（AS）concentration was 10 mg/L.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第4期93-97,共5页
Biotechnology Bulletin
基金
中央级公益性科研院所基本科研业务费专项资金项目(2006-1-01)
关键词
紫花苜蓿
遗传转化
农杆菌介导
Alfalfa Genetic transformation Agrobacterium-mediated
