水杨酸浸种处理对黑麦草种子萌发及幼苗抗旱性的影响

Effects of soaking seeds with salicylic acid on seed germination and drought resistance of Lolium perenne seedlings

用0.25、0.50和0.75 mmol/L水杨酸（SA）对黑麦草‘托亚’（Lolium perenne‘Taya’）进行浸种处理,研究不同浓度SA对种子萌发的影响及0.50 mmol/L SA浸种处理对黑麦草幼苗抗旱性的影响。结果表明,0.25~0.75 mmol/L SA浸种预处理有效地提高了黑麦草种子的发芽率和发芽势以及幼苗的鲜质量和干质量,以0.50mmol/L SA处理促进发芽的效果最为显著。水分胁迫下,0.50 mmol/L SA浸种抑制黑麦草幼苗质膜透性的增加和丙二醛的累积,促进脯氨酸含量的增加和超氧化物歧化酶、过氧化物酶活性的升高,过氧化氢酶活性也表现出相对的稳定性。因此,SA浸种对水分胁迫具有一定的缓解效应。

The 0.25,0.50 and 0.75 mmol/L salicylic acid（SA） were used to soak seeds of Lolium perenne for determining the effects of soaking seeds on seed germination and drought resistance of seedlings.This study showed that the seeds of L.perenne treated by 0.25~0.75 mmol/L SA increased the germination percentage,germination energy of the seeds and the fresh and dry weights of seedlings,and seed germination was the biggest when seeds were treated by 0.5 mmol/L SA.Under water stress,0.5 mmol/L SA inhibited the permeability of plasma membrane,increased the accumulation of Malondiald Ehyde（MDA） and proline content,encouraged the activities of Superoxide Dismutase（SOD） and Peroxidase（POD） to increase in the L.perenne,and kept the activity of Catase（CAT） stable.This study suggested that SA could mitigate the water stress.