莪术黄芪超滤膜提取物诱导人SGC7901胃癌细胞凋亡的研究

Study on the Ultra-filtration Extract from Rhizoma Curcumae and Astragalus Membranaceous in Inducing SGC7901 Cell Apoptosis

目的:观察莪术黄芪超滤膜提取物对人SGC7901细胞是否具有凋亡诱导作用。方法:四氮唑蓝比色法(MTT法)观察莪术黄芪超滤膜提取物对人SGC7901细胞细胞的抑制率;应用流式细胞术,DNA凝胶电泳方法检测细胞凋亡的发生。结果:(1)莪术黄芪超滤膜提取物对人SGC7901细胞的生长有明显的抑制作用,与对照组相比有显著性差异(P<0.01),抑制作用与剂量及时间呈显著性正相关。(2)莪术、黄芪超滤膜提取物诱导人SGC7901细胞发生凋亡,1.6g/L处理,72h效果最佳。结论:莪术黄芪超滤膜提取物抑制人SGC7901细胞生长并促其凋亡。

Objective：To observe the effect of the ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous on inducing human SGC7901 cell apoptosis.Methods：SGC7901 cell proliferation was detected by MTT colorimetry.Apoptosis was observed by flow cytometry and agarose gel electrophoresis.Results：The ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous could significantly inhibit SGC7901 cell proliferation and there was a significant difference compared with that in the control groups（P0.01）.There was a remarkable positive correlation between drug concentration of time and the inhibitory rates;The ultra-filtration extract from Rhizoma Curcumae and Astragalus Membranaceous could induce SGC7901 cell apoptosis.An optimal result is achieved with 1.6g/L for 72h.Conclusion：The ultra-filtration extract from Rhizoma Curcumae and Astragalus Memb-ranaceous could induce SGC7901 cell apoptosis.