指标的检测对糖尿病大鼠表征的影响

The Influence of Detection of Index on Characteristics of Diabetic Rats

目的:观察定期检测胰岛素对糖尿病大鼠表征的影响。方法:链脲佐菌素(STZ)腹腔注射50mg/kg建立糖尿病模型,观察4周。模型1组(M1组)与正常1组(N1组)每周尾静脉采血0.5mL测试胰岛素;模型2组(M2组)与正常2组(N2组)在处死后采血测试胰岛素。定期观察大鼠一般状况,检测大鼠体重、血糖、饮水量、尿量、体温、旷场活动、抓力。结果:分别与N1、N2组比较,M1、M2组大鼠体重及抓力下降、血糖升高、饮水量及尿量增加,差异有统计学意义(P<0.05);M2组大鼠体温下降,差异有统计学意义(P<0.05),毛、尾巴、爪光泽尚可;M1组大鼠体温改变不明显,毛枯槁、尾巴严重结痂、爪脱屑。结论:M1、M2组糖尿病大鼠具有不同的表征,可能与M1组定期尾静脉采血有关,提示指标检测过程会影响动物的表征。

Objective：To observe the influence of detection of insulin timely on characteristics of diabetic rats.Methods：Diabetic rats were induced with streptozotocin intraperitoneally injected.Rats in model 1 group and normal 1 group received hemospasia from tail vein to detect insulin every week.Rats in model 2 group and normal 2 group detected insulin after death.The rats were observed for four weeks,their general condition,body weight,blood glucose,drinking amount,urine volume,temperature,open-field test,grasp force were tested.Results：Compared with N1 or N2 group,body weight and grasp force of rats in M1 or M2 group reduced,blood glucose increased,drinking amount and urine volume rose.In rats of M2 group,the temperature and movements reduced;the burnish of hair,tail and claw were acceptable.In rats of M1 group,the temperature didn＇t change obviously;the hair were withered and yellow,the tails scabbed,the claws desquamated.Conclusion：The diabetic rats in M1、M2 group show different characteristics,maybe this is related to hemospasia from tail vein timely.And the detection of index could affect the characteristics of diabetic rats.